Sheep IgG anti-Mouse IgG (H+L)-HRPO, MinX none

Product Overview

• Article Name: Sheep IgG anti-Mouse IgG (H+L)-HRPO, MinX none
• Biozol Catalog Number: ROC-610-603-002
• Supplier Catalog Number: 610-603-002
• Alternative Catalog Number: ROC-610-603-002
• Manufacturer: Rockland Immunochemicals
• Host: Sheep
• Category: Antikörper
• Application: ELISA,IHC,WB
• Species Reactivity: Mouse
• Immunogen: Anti-Mouse IgG [H&L] was produced by repeated immunization with Mouse IgG whole molecule in sheep.
• Conjugation: HRPO
• Alternative Names: sheep anti-Mouse IgG heavy and light chain peroxidase conjugated Antibody, sheep anti-Mouse IgG HRP secondary Antibody, sh anti-ms IgG HRP antibody
• Format: IgG
• Target Specificity: IgG (H+L)
• Cross-Adsorption (MinX): no cross-adsorbtion

Product Properties

• Clonality: Polyclonal
• Concentration: 2.0 mg/mL by UV absorbance at 280 nm
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Lyophilized
• Target: Mouse
• Antibody Type: Secondary Antibody

Application Notes

• Application Dilute: ELISA: 1:100,000 - 1:200,000, IHC: 1:500 - 1:2,500, WB: 1:1,000 - 1:5,000
• Application Notes: Anti-Mouse IgG Peroxidase Conjugated Antibody has been tested by ELISA and is suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic ass

Images

Western Blot of Peroxidase conjugated Sheep anti-Mouse IgG antibody. Lane 1: Mouse IgG. Lane 2: None. Load: 100 ng per lane. Primary antibody: none. Secondary antibody: Peroxidase sheep secondary antibody at 1:1,000 for 60 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 55 and 28 kDa, 55 and 28 kDa for Mouse IgG. Other band(s): none.