Donkey IgG anti-Mouse IgG (H+L)-HRPO, MinX Bo,Ck,Go,Gp,Hm,Ho,Hu,Rb,Rt,Sh, Polyclonal

Product Overview

• Article Name: Donkey IgG anti-Mouse IgG (H+L)-HRPO, MinX Bo,Ck,Go,Gp,Hm,Ho,Hu,Rb,Rt,Sh, Polyclonal
• Biozol Catalog Number: ROC-610-703-124
• Supplier Catalog Number: 610-703-124
• Alternative Catalog Number: ROC-610-703-124
• Manufacturer: Rockland Immunochemicals
• Host: Donkey
• Category: Antikörper
• Application: ELISA,IHC,WB
• Species Reactivity: Mouse
• Immunogen: Anti-Mouse IgG whole molecule was produced by repeated immunization with Mouse IgG whole molecule in donkey.
• Conjugation: HRPO
• Alternative Names: Donkey Anti-Mouse IgG Antibody Peroxidase Conjugation, Donkey Anti-Mouse IgG HRP Conjugated Antibody
• Format: IgG
• Target Specificity: IgG (H+L)
• Cross-Adsorption (MinX): Bovine,Gallus,Goat,Guinea pig,Hamster (all),Equine,Human,Rabbit,Rat,Sheep

Product Properties

• Clonality: Polyclonal
• Concentration: 1.0 mg/mL by UV absorbance at 280 nm
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Lyophilized
• Target: Mouse
• Antibody Type: Secondary Antibody

Application Notes

• Application Dilute: ELISA: 1:20,000 - 1:80,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:20,000
• Application Notes: Anti-Mouse IgG Peroxidase Conjugated Antibody has been tested by ELISA and western blot and is suitable for immunoblotting (western or dot blot), ELISA, immunoelectron microscopy and immunohistochemistry as well as other peroxidase antibody-based enzymat

Images

Western Blot of Peroxidase Donkey Anti-Mouse IgG Pre-Adsorbed secondary antibody. Lane 1: Mouse IgG. Lane 2: None. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: Peroxidase donkey secondary antibody at 1:1,000 for 60 min at RT. Blocking: MB-070 for 30 min at RT. Predicted/Observed size: 25 & 55 kDa, 25 & 55 kDa for Mouse IgG. Other band(s): None.