Donkey IgG anti-Mouse IgG (H+L)-Alk. Phos., MinX Bo,Ck,Go,Gp,Hm,Ho,Hu,Rb,Rt,Sh, ALP, Polyclonal , AP

Product Overview

• Article Name: Donkey IgG anti-Mouse IgG (H+L)-Alk. Phos., MinX Bo,Ck,Go,Gp,Hm,Ho,Hu,Rb,Rt,Sh, ALP, Polyclonal , AP
• Biozol Catalog Number: ROC-610-705-124
• Supplier Catalog Number: 610-705-124
• Alternative Catalog Number: ROC-610-705-124
• Manufacturer: Rockland Immunochemicals
• Host: Donkey
• Category: Antikörper
• Application: ELISA,IHC,WB
• Species Reactivity: Mouse
• Immunogen: Mouse IgG whole molecule
• Conjugation: Alk. Phos.
• Alternative Names: Donkey anti-Mouse IgG alkaline phosphatase conjugated Antibody, Donkey anti-Mouse IgG Antibody alk phos conjugation
• Format: IgG
• Target Specificity: IgG (H+L)
• Cross-Adsorption (MinX): Bovine,Gallus,Goat,Guinea pig,Hamster (all),Equine,Human,Rabbit,Rat,Sheep

Product Properties

• Clonality: Polyclonal
• Concentration: 1.0 mg/mL by UV absorbance at 280 nm
• Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol, pH 8.0
• Form: Liquid (sterile filtered)
• Target: Mouse
• Antibody Type: Secondary Antibody

Application Notes

• Application Dilute: ELISA: 1:2,000 - 1:20,000, IHC: 1:500 - 1:2,500, WB: 1:1,000 - 1:5,000
• Application Notes: Anti-Mouse IgG Alkaline Phosphatase Antibody has been tested by ELISA and dot blot and is suitable for immunoblotting (western or dot blot), ELISA, immunohistochemistry as well as other alkaline phosphatase-antibody based enzymatic assays requiring lot-t

Images

ELISA results of purified Donkey Anti-Mouse IgG Alkaline Phosphatase Conjugated Antibody tested against purified Mouse IgG. (Blue Line). Each well was coated in duplicate with 1.0 µg of antigen. The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using Blocking buffer (p/n MB-060-1000) and TMB substrate (p/n TMBE-1000).