Donkey IgG anti-Mouse IgG (H+L)-Biotin, MinX none

Product Overview

• Article Name: Donkey IgG anti-Mouse IgG (H+L)-Biotin, MinX none
• Biozol Catalog Number: ROC-610-706-002
• Supplier Catalog Number: 610-706-002
• Alternative Catalog Number: ROC-610-706-002
• Manufacturer: Rockland Immunochemicals
• Host: Donkey
• Category: Antikörper
• Application: ELISA,IHC,WB
• Species Reactivity: Mouse
• Immunogen: Mouse IgG whole molecule
• Conjugation: Biotin
• Alternative Names: Donkey anti-Mouse IgG Biotin Conjugated Antibody, Donkey anti Mouse IgG Antibody Biotin Conjugation
• Format: IgG
• Target Specificity: IgG (H+L)
• Cross-Adsorption (MinX): no cross-adsorbtion

Product Properties

• Clonality: Polyclonal
• Concentration: 2.0 mg/mL by UV absorbance at 280 nm
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Lyophilized
• Target: Mouse
• Antibody Type: Secondary Antibody

Application Notes

• Application Dilute: ELISA: 1:20,000 - 1:100,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000
• Application Notes: Anti-Mouse IgG Biotin Antibody has been tested by ELISA and is assayed against 1.0 ug of Mouse IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin S000-03 and ABTS (2,2-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code ABTS-

Images

ELISA results of purified Donkey anti-Mouse IgG Biotin conjugated Antibody tested against Mouse IgG. Each well was coated in duplicate with 1.0 µg of Mouse IgG (purple line). The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using Blocking buffer MB-060-1000, Streptavidin HRP conjugate, and TMB-1000 substrate.