Mouse IgG (H&L) Antibody CY2 Conjugated Pre-Adsorbed, Cy2, Donkey, Polyclonal

Product Overview

• Article Name: Mouse IgG (H&L) Antibody CY2 Conjugated Pre-Adsorbed, Cy2, Donkey, Polyclonal
• Biozol Catalog Number: ROC-610-711-124
• Supplier Catalog Number: 610-711-124
• Alternative Catalog Number: ROC-610-711-124
• Manufacturer: Rockland Immunochemicals
• Host: Donkey
• Category: Antikörper
• Species Reactivity: Mouse
• Immunogen: Mouse IgG whole molecule
• Conjugation: Cy2
• Alternative Names: Donkey Anti-Mouse IgG (H&L) Antibody CY2 Conjugated Pre-Adsorbed, Donkey Anti Mouse IgG Antibody CY2 Conjugated

Product Properties

• Clonality: Polyclonal
• Concentration: 1.0 mg/mL by UV absorbance at 280 nm
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Lyophilized
• Target: Mouse
• Antibody Type: Secondary Antibody

Application Notes

• Application Dilute: ELISA: 1:10,000 - 1:50,000, FLISA: 1:10,000 - 1:50,000, Flow Cytometry: 1:500 - 1:2,500, IF Microscopy: 1:1,000 - 1:5,000, WB: User Optimized
• Application Notes: Cy2 Conjugated Secondary Antibody is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing vario

Images

nBAF subunits are depleted from motor neurons in multiple forms of familial and sporadic ALS. Cross-sections of autopsied spinal cord from individuals dying of non-ALS causes (control), familial ALS owing toC9ORF72G4C2nucleotide expansion, or sporadic ALS with no known mutation were labelled with antibodies to the nBAF subunits (A) Brg1 (red). Sections were labelled with SMI32 recognizing the high molecular weight neurofilament subunit (NFH) to identify motor neurons (green) using anti-mouse Cy2-IgG (p/n 610-711-124) and DAPI to label nuclei (blue). Arrow heads point to nuclei of motor neurons. Arrows point to accumulations of autofluorescent lipofuscin. Illustrated are representative images from examination of multiple sections of three cases per condition. MeanSD: 1000% (n=3 control cases) and 129% (n=8 pooled C9ORF72 and sALS cases. Significant difference between control and ALS case groups,p=0.0001). Scale bar=16µm. Fig 6. PMID: 28973294.