Rabbit IgG Fc Antibody Texas Red(TM) Conjugated Pre-Adsorbed, Goat, Polyclonal

Product Overview

• Article Name: Rabbit IgG Fc Antibody Texas Red(TM) Conjugated Pre-Adsorbed, Goat, Polyclonal
• Biozol Catalog Number: ROC-611-1923
• Supplier Catalog Number: 611-1923
• Alternative Catalog Number: ROC-611-1923
• Manufacturer: Rockland Immunochemicals
• Host: Goat
• Category: Antikörper
• Application: DOT
• Species Reactivity: Rabbit
• Immunogen: Rabbit IgG F(c) fragment
• Conjugation: Texas Red
• Alternative Names: Goat Anti-Rabbit IgG F(c) Texas Red(TM) Conjugated Antibody, Goat Anti-Rabbit IgG Fc Fragment Antibody Texas Red(TM) conjugation, Goat Anti Rabbit IgG Fc Antibody Texas Red(TM) conjugated

Product Properties

• Clonality: Polyclonal
• Concentration: 2.0 mg/mL by UV absorbance at 280 nm
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Lyophilized
• Target: Rabbit
• Antibody Type: Secondary Antibody

Application Notes

• Application Dilute: FLISA: 1:10,000 - 1:50,000, Flow Cytometry: 1:500 - 1:2,500, IF Microscopy: 1:1,000 - 1:5,000
• Application Notes: Anti-Rabbit IgG F(c) Texas Red antibody has been tested by dot blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, includi

Images

Western Blot of Anti-Rabbit IgG F(c) (GOAT) Antibody (p/n 611-1103). Lane M: 3 µl Molecular Ladder. Lane 1: Rabbit IgG whole molecule (p/n 011-0102). Lane 2: Rabbit IgG F(ab) Fragment (p/n 011-0105). Lane 3: Rabbit IgG F(c) Fragment (p/n 010-0103). Lane 4: Rabbit IgM Whole Molecule (p/n 011-0107). Lane 5: Normal Rabbit Serum (p/n B309). All samples were reduced. Load: 50 ng of IgG, F(ab), IgM and Serum, 100 ng of F(c). Block: MB-070 for 30 min at RT. Primary Antibody: Anti-Rabbit IgG F(c) (GOAT) Antibody (p/n 611-1103) 1:2,000 for 60 min at RT. Secondary antibody: Anti-Goat IgG (DONKEY) Peroxidase Conjugated Antibody (p/n CUST10) 1:40,000 in MB-070 for 30 min at RT. Predicted/Observed Size: 25 and 50 kDa for Rabbit IgG and Serum, 25 kDa for F(c) and F(ab), 70 and 23 kDa for IgM. Rabbit F(c) migrates slightly higher.