Rabbit IgG (H&L) Antibody Fluorescein Conjugated Pre-Adsorbed, FITC, Guinea pig, Polyclonal

Product Overview

• Article Name: Rabbit IgG (H&L) Antibody Fluorescein Conjugated Pre-Adsorbed, FITC, Guinea pig, Polyclonal
• Biozol Catalog Number: ROC-611-202-122
• Supplier Catalog Number: 611-202-122
• Alternative Catalog Number: ROC-611-202-122
• Manufacturer: Rockland Immunochemicals
• Host: Guinea pig
• Category: Antikörper
• Species Reactivity: Rabbit
• Immunogen: Rabbit IgG whole molecule
• Conjugation: FITC
• Alternative Names: Guinea Pig Anti-Rabbit IgG Fluorescein Conjugated Antibody, Guinea Pig Anti Rabbit IgG Antibody FITC Conjugation

Product Properties

• Clonality: Polyclonal
• Concentration: 1.0 mg/mL by UV absorbance at 280 nm
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Lyophilized
• Target: Rabbit
• Antibody Type: Secondary Antibody

Application Notes

• Application Dilute: FLISA: 1:10,000 - 1:50,000, Flow Cytometry: 1:500 - 1:2,500, IF Microscopy: 1:1,000 - 1:5,000
• Application Notes: This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platfor

Images

Western Blot of Anti-Rabbit IgG (H&L) (GUINEA PIG) Antibody (Min X Hu, Gt, Ms Serum Proteins) (p/n 611-201-122). Lane M: 3 µl Molecular Ladder. Lane 1: Rabbit IgG whole molecule (p/n 011-0102). Lane 2: Rabbit IgG F(ab) Fragment (p/n 011-0105). Lane 3: Rabbit IgG F(c) Fragment (p/n 010-0103). All samples were reduced. Load: 50 ng per lane. Block: MB-070 for 30 min at RT. Primary Antibody: Anti-Rabbit IgG (H&L) (GUINEA PIG) Antibody (Min X Hu, Gt, Ms Serum Proteins) (p/n 611-201-122) 1:500 for 60 min at RT. Secondary antibody: Anti-Guinea Pig IgG (GOAT) Peroxidase Conjugated Antibody (p/n 606-103-129) 1:40,000 in MB-070 for 30 min at RT. Predicted/Observed Size: 25 and 50 kDa for Rabbit IgG and Serum, 25 kDa for F(c) and F(ab). Rabbit F(c) migrates slightly higher.