Donkey IgG anti-Rabbit IgG (H+L)-Alk. Phos., MinX Bo,Ck,Go,Gp,Hm,Ho,Hu,Ms,Rt,Sh, ALP, Polyclonal , AP

Product Overview

• Article Name: Donkey IgG anti-Rabbit IgG (H+L)-Alk. Phos., MinX Bo,Ck,Go,Gp,Hm,Ho,Hu,Ms,Rt,Sh, ALP, Polyclonal , AP
• Biozol Catalog Number: ROC-611-705-127
• Supplier Catalog Number: 611-705-127
• Alternative Catalog Number: ROC-611-705-127
• Manufacturer: Rockland Immunochemicals
• Host: Donkey
• Category: Antikörper
• Application: ELISA,IHC,WB
• Species Reactivity: Rabbit
• Immunogen: Anti-Rabbit IgG (H&L) was produced by repeated immunization with Rabbit IgG whole molecule in donkey
• Conjugation: Alk. Phos.
• Alternative Names: Donkey anti-Rabbit IgG Alkaline Phosphatase Conjugated Antibody, Donkey anti-Rabbit IgG Antibody Alkaline Phosphatase Conjugation
• Format: IgG
• Target Specificity: IgG (H+L)
• Cross-Adsorption (MinX): Bovine,Gallus,Goat,Guinea pig,Hamster (all),Equine,Human,Mouse,Rat,Sheep

Product Properties

• Clonality: Polyclonal
• Concentration: 1.0 mg/mL by UV absorbance at 280 nm
• Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol, pH 8.0
• Form: Liquid (sterile filtered)
• Target: Rabbit
• Antibody Type: Secondary Antibody

Application Notes

• Application Dilute: ELISA: 1:10,000 - 1:20,000, IHC: 1:400 - 1:2,000, WB: 1:1,000 - 1:5,000
• Application Notes: Anti-Rabbit IgG (H&L) Alkaline Phosphatase Antibody has been tested by dot blot and ELISA and is suitable for immunoblotting (western or dot blot), ELISA, and immunohistochemistry assays requiring lot-to-lot consistency.

Images

Western Blot of Anti-Rabbit IgG (H&L) (DONKEY) Antibody (Min X Bv Ch Gt GP Ham Hs Hu Ms Rt & Sh Serum Proteins) (p/n 611-701-127). Lane M: 3 µl Molecular Ladder. Lane 1: Rabbit IgG whole molecule (p/n 011-0102). Lane 2: Rabbit IgG F(ab) Fragment (p/n 011-0105). Lane 3: Rabbit IgG F(c) Fragment (p/n 010-0103). Lane 4: Rabbit IgM Whole Molecule (p/n 011-0107). Lane 5: Normal Rabbit Serum (p/n B309). All samples were reduced. Load: 50 ng of IgG, F(ab), F(c) and Serum, 25 ng of IgM. Block: MB-070 for 30 min at RT. Primary Antibody: Anti-Rabbit IgG (H&L) (DONKEY) Antibody (Min X Bv Ch Gt GP Ham Hs Hu Ms Rt & Sh Serum Proteins) (p/n 611-701-127) 1:7,500 for 60 min at RT. Secondary antibody: Anti-Donkey IgG (GOAT) Peroxidase Conjugated Antibody (p/n 616-1302) 1:40,000 in MB-070 for 30 min at RT. Predicted/Observed Size: 25 and 50 kDa for Rabbit IgG and Serum, 25 kDa for F(c) and F(ab), 70 and 23 kDa for IgM. Rabbit F(c) migrates slightly higher.