Donkey IgG anti-Rabbit IgG (H+L)-Biotin, MinX Bo,Ck,Go,Gp,Hm,Ho,Hu,Ms,Rt,Sh, Polyclonal

Product Overview

• Article Name: Donkey IgG anti-Rabbit IgG (H+L)-Biotin, MinX Bo,Ck,Go,Gp,Hm,Ho,Hu,Ms,Rt,Sh, Polyclonal
• Biozol Catalog Number: ROC-611-706-127
• Supplier Catalog Number: 611-706-127
• Alternative Catalog Number: ROC-611-706-127
• Manufacturer: Rockland Immunochemicals
• Host: Donkey
• Category: Antikörper
• Application: ELISA,IHC,WB
• Species Reactivity: Rabbit
• Immunogen: Rabbit IgG whole molecule
• Conjugation: Biotin
• Alternative Names: Donkey anti-Rabbit IgG Biotin Conjugated Antibody, Donkey anti-Rabbit IgG Antibody Biotin Conjugation
• Format: IgG
• Target Specificity: IgG (H+L)
• Cross-Adsorption (MinX): Bovine,Gallus,Goat,Guinea pig,Hamster (all),Equine,Human,Mouse,Rat,Sheep

Product Properties

• Clonality: Polyclonal
• Concentration: 1.0 mg/mL by UV absorbance at 280 nm
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Lyophilized
• Target: Rabbit
• Antibody Type: Secondary Antibody

Application Notes

• Application Dilute: ELISA: 1:20,000 - 1:60,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000
• Application Notes: Anti-Rabbit IgG (H&L) Biotin Antibody has been tested by ELISA and is assayed against 1.0 ug of Rabbit IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin S000-03 and ABTS (2,2-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code

Images

Western Blot of Anti-Rabbit IgG (H&L) (DONKEY) Antibody (Min X Bv Ch Gt GP Ham Hs Hu Ms Rt & Sh Serum Proteins) (p/n 611-701-127). Lane M: 3 µl Molecular Ladder. Lane 1: Rabbit IgG whole molecule (p/n 011-0102). Lane 2: Rabbit IgG F(ab) Fragment (p/n 011-0105). Lane 3: Rabbit IgG F(c) Fragment (p/n 010-0103). Lane 4: Rabbit IgM Whole Molecule (p/n 011-0107). Lane 5: Normal Rabbit Serum (p/n B309). All samples were reduced. Load: 50 ng of IgG, F(ab), F(c) and Serum, 25 ng of IgM. Block: MB-070 for 30 min at RT. Primary Antibody: Anti-Rabbit IgG (H&L) (DONKEY) Antibody (Min X Bv Ch Gt GP Ham Hs Hu Ms Rt & Sh Serum Proteins) (p/n 611-701-127) 1:7,500 for 60 min at RT. Secondary antibody: Anti-Donkey IgG (GOAT) Peroxidase Conjugated Antibody (p/n 616-1302) 1:40,000 in MB-070 for 30 min at RT. Predicted/Observed Size: 25 and 50 kDa for Rabbit IgG and Serum, 25 kDa for F(c) and F(ab), 70 and 23 kDa for IgM. Rabbit F(c) migrates slightly higher.