Donkey IgG anti-Rabbit IgG (H+L)-unconj., MinX none

Product Overview

• Article Name: Donkey IgG anti-Rabbit IgG (H+L)-unconj., MinX none
• Biozol Catalog Number: ROC-611-7102
• Supplier Catalog Number: 611-7102
• Alternative Catalog Number: ROC-611-7102
• Manufacturer: Rockland Immunochemicals
• Host: Donkey
• Category: Antikörper
• Application: ELISA,IHC,WB
• Species Reactivity: Rabbit
• Immunogen: Rabbit IgG whole molecule
• Conjugation: Unconjugated
• Alternative Names: Donkey Anti-Rabbit IgG Antibody, Donkey Anti Rabbit IgG Antibody
• Format: IgG
• Target Specificity: IgG (H+L)
• Cross-Adsorption (MinX): no cross-adsorbtion

Product Properties

• Clonality: Polyclonal
• Concentration: 2.20 mg/mL by UV absorbance at 280 nm
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Liquid (sterile filtered)
• Target: Rabbit
• Antibody Type: Secondary Antibody

Application Notes

• Application Dilute: ELISA: 1:40,000 - 1:160,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000
• Application Notes: Anti-Rabbit IgG antibody has been tested by ELISA and is suitable for ELISA, western blot, and immunohistochemistry, as well as other assays requiring lot-to-lot consistency.

Images

Western Blot of Anti-Rabbit IgG (H&L) (DONKEY) Antibody Peroxidase Conjugated (p/n 611-7302). Lane M: 3 µl Molecular Ladder. Lane 1: Rabbit IgG whole molecule (p/n 011-0102). Lane 2: Rabbit IgG F(ab) Fragment (p/n 011-0105). Lane 3: Rabbit IgG F(c) Fragment (p/n 010-0103). Lane 4: Rabbit IgM Whole Molecule (p/n 011-0107). Lane 5: Normal Rabbit Serum (p/n B309). All samples were reduced. Load: 50 ng per lane. Block: MB-070 for 30 min at RT. Primary Antibody: Anti-Rabbit IgG (H&L) (DONKEY) Antibody Peroxidase Conjugated (p/n 611-7302) 1:5,000 for 60 min at RT. Secondary antibody: None. Predicted/Observed Size: 25 and 50 kDa for Rabbit IgG and Serum, 25 kDa for F(c) and F(ab), 70 and 23 kDa for IgM. Rabbit F(c) migrates slightly higher.