Goat IgG anti-Rat IgG (H+L)-RPE, MinX Bo,Ck,Go,Gp,Hm,Ho,Hu,Ms,Rb,Sh, Polyclonal

Product Overview

• Article Name: Goat IgG anti-Rat IgG (H+L)-RPE, MinX Bo,Ck,Go,Gp,Hm,Ho,Hu,Ms,Rb,Sh, Polyclonal
• Biozol Catalog Number: ROC-612-108-120
• Supplier Catalog Number: 612-108-120
• Alternative Catalog Number: ROC-612-108-120
• Manufacturer: Rockland Immunochemicals
• Host: Goat
• Category: Antikörper
• Application: FACS,IF
• Species Reactivity: Rat
• Immunogen: Anti-Rat IgG whole molecule was produced by repeated immunization with Rat IgG whole molecule in goat.
• Conjugation: RPE
• Alternative Names: Goat Anti-Rat IgG phycoerythrin Conjugated Antibody, Goat Anti-Rat IgG Antibody PE Conjugation
• Format: IgG
• Target Specificity: IgG (H+L)
• Cross-Adsorption (MinX): Bovine,Gallus,Goat,Guinea pig,Hamster (all),Equine,Human,Mouse,Rabbit,Sheep

Product Properties

• Clonality: Polyclonal
• Concentration: 0.5 mg/mL by absorbance = 82.0 at 565 nm
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Lyophilized
• Target: Rat
• Antibody Type: Secondary Antibody

Application Notes

• Application Dilute: Flow Cytometry: 1:100 - 1:250, IF Microscopy: 1:100 - 1:250
• Application Notes: Anti-Rat IgG Phycoerythrin has been tested by western blot and is suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated bin

Images

Western Blot of Anti-Rat IgG (H&L) (GOAT) Antibody (Min X Bv Ch Gt GP Ham Hs Hu Ms Rb & Sh Serum Proteins) (p/n 612-101-120). Lane M: 3 µl Molecular Ladder. Lane 1: Rat IgG whole molecule (p/n 012-0102). Lane 2: Rat IgG F(c) Fragment (p/n 012-0103). Lane 3: Rat IgG Fab Fragment (p/n 012-0105). Lane 4: Rat IgM Whole Molecule (p/n 012-0107). Lane 5: Rat Serum (p/n D310-05). All samples were reduced. Load: 50 ng per lane. Block: MB-070 for 30 min at RT. Primary Antibody: Anti-Rat IgG (H&L) (GOAT) Antibody (Min X Bv Ch Gt GP Ham Hs Hu Ms Rb & Sh Serum Proteins) (p/n 612-101-120) 1:1,000 for 60 min at RT. Secondary Antibody: Anti-Goat IgG (DONKEY) Peroxidase Conjugated Antibody (p/n CUST10) 1:40,000 in MB-070 for 30 min at RT. Predicted/Observed Size: 25 and 55 kDa for Rat IgG and Serum, 25 kDa for F(c) and Fab, 78 and 25 kDa for IgM. Rat F(c) migrates slightly higher.