Rat IgG (H&L) Antibody CY2 Conjugated Pre-Adsorbed, Cy2, Goat, Polyclonal

Product Overview

• Article Name: Rat IgG (H&L) Antibody CY2 Conjugated Pre-Adsorbed, Cy2, Goat, Polyclonal
• Biozol Catalog Number: ROC-612-111-120
• Supplier Catalog Number: 612-111-120
• Alternative Catalog Number: ROC-612-111-120
• Manufacturer: Rockland Immunochemicals
• Host: Goat
• Category: Antikörper
• Species Reactivity: Rat
• Immunogen: Rat IgG whole molecule
• Conjugation: Cy2
• Alternative Names: Goat Anti Rat IgG CY2 Conjugated Antibody, Goat Anti-Rat IgG Antibody CY2 Conjugation

Product Properties

• Clonality: Polyclonal
• Concentration: 1.0 mg/mL by UV absorbance at 280 nm
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Lyophilized
• Target: Rat
• Antibody Type: Secondary Antibody

Application Notes

• Application Dilute: ELISA: 1:10,000 - 1:50,000, FLISA: 1:10,000 - 1:50,000, Flow Cytometry: 1:500 - 1:2,500, IF Microscopy: 1:1,000 - 1:5,000, WB: User Optimized
• Application Notes: Cy2 Conjugated Secondary Antibody to Rat IgG is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, util

Images

Western Blot of Anti-Rat IgG (H&L) (GOAT) Antibody (Min X Bv Ch Gt GP Ham Hs Hu Ms Rb & Sh Serum Proteins) (p/n 612-101-120). Lane M: 3 µl Molecular Ladder. Lane 1: Rat IgG whole molecule (p/n 012-0102). Lane 2: Rat IgG F(c) Fragment (p/n 012-0103). Lane 3: Rat IgG Fab Fragment (p/n 012-0105). Lane 4: Rat IgM Whole Molecule (p/n 012-0107). Lane 5: Rat Serum (p/n D310-05). All samples were reduced. Load: 50 ng per lane. Block: MB-070 for 30 min at RT. Primary Antibody: Anti-Rat IgG (H&L) (GOAT) Antibody (Min X Bv Ch Gt GP Ham Hs Hu Ms Rb & Sh Serum Proteins) (p/n 612-101-120) 1:1,000 for 60 min at RT. Secondary Antibody: Anti-Goat IgG (DONKEY) Peroxidase Conjugated Antibody (p/n CUST10) 1:40,000 in MB-070 for 30 min at RT. Predicted/Observed Size: 25 and 55 kDa for Rat IgG and Serum, 25 kDa for F(c) and Fab, 78 and 25 kDa for IgM. Rat F(c) migrates slightly higher.