Goat IgG anti-Rat IgG (H+L)-Alk. Phos., MinX none, ALP, Polyclonal , AP

Catalog Number: ROC-612-1502
Article Name: Goat IgG anti-Rat IgG (H+L)-Alk. Phos., MinX none, ALP, Polyclonal , AP
Biozol Catalog Number: ROC-612-1502
Supplier Catalog Number: 612-1502
Alternative Catalog Number: ROC-612-1502
Manufacturer: Rockland Immunochemicals
Host: Goat
Category: Antikörper
Application: ELISA,IHC,WB
Species Reactivity: Rat
Immunogen: Rat IgG whole molecule
Conjugation: Alk. Phos.
Alternative Names: Goat anti-Rat IgG Alkaline Phosphatase Conjugated Antibody, Goat anti-Rat IgG Antibody Alk Phos Conjugation
Format: IgG
Target Specificity: IgG (H+L)
Cross-Adsorption (MinX): no cross-adsorbtion
Clonality: Polyclonal
Concentration: 1.0 mg/mL by UV absorbance at 280 nm
Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol, pH 8.0
Form: Liquid (sterile filtered)
Target: Rat
Antibody Type: Secondary Antibody
Application Dilute: ELISA: 1:50,000 - 1:90,000, IHC: 1:200 - 1:1,000, WB: 1:500 - 1:2,500
Application Notes: Anti-Rat IgG Alkaline Phosphatase antibody has been tested by ELISA, dot blot, and western blot and is suitable for use in immunohistochemistry. Specific conditions for reactivity should be optimized by the end user.
Western Blot of Alkaline Phosphatase Conjugated Goat anti-Rat IgG antibody. Lane 1: Rat IgG. Lane 2: none. Load: 50 ng per lane. Primary antibody: none. Secondary antibody: Alkaline Phosphatase rat secondary antibody at 1:1,000 for 60 min at RT. Block: MB-070 for 30 min RT. Predicted/Observed size: 55 kDa, 28 kDa for Rat IgG. Other band(s): none.