Goat IgG anti-Rat IgG (H+L)-Biotin, MinX none

Product Overview

• Article Name: Goat IgG anti-Rat IgG (H+L)-Biotin, MinX none
• Biozol Catalog Number: ROC-612-1602
• Supplier Catalog Number: 612-1602
• Alternative Catalog Number: ROC-612-1602
• Manufacturer: Rockland Immunochemicals
• Host: Goat
• Category: Antikörper
• Application: ELISA,IHC,WB
• Species Reactivity: Rat
• Immunogen: Rat IgG whole molecule
• Conjugation: Biotin
• Alternative Names: Goat anti-Rat IgG biotin Conjugated Antibody, Goat anti-Rat IgG Antibody biotin Conjugation
• Format: IgG
• Target Specificity: IgG (H+L)
• Cross-Adsorption (MinX): no cross-adsorbtion

Product Properties

• Clonality: Polyclonal
• Concentration: 2.0 mg/mL by UV absorbance at 280 nm
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Lyophilized
• Target: Rat
• Antibody Type: Secondary Antibody

Application Notes

• Application Dilute: ELISA: 1:20,000 - 1:100,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000
• Application Notes: Anti-RAT IgG Biotin Conjugated antibody has been tested by ELISA and western blot and is suitable for immunoassays where specificity to the Rat immunoglobulin heavy and or light chain regions is desired. Anti-Rat antibody has been assayed against 1.0 µg

Images

Western Blot of Goat anti-Rat IgG (H&L) Antibody Biotin Conjugated. Lane 1: Rat IgG. Load: 100 ng per lane. Primary antibody: Rat IgG (H&L) Antibody Biotin Conjugated at 1:1000 for 60 min RT. Secondary antibody: HRP Conjugated Streptavidin at 1:40,000 for 30 min at RT. Block: MB-070 for 30 min at RT.

Western Blot of Anti-Rat IgG (H&L) (GOAT) Antibody (p/n 612-1102). Lane M: 3 µl Molecular Ladder. Lane 1: Rat IgG whole molecule (p/n 012-0102). Lane 2: Rat IgG F(c) Fragment (p/n 012-0103). Lane 3: Rat IgG Fab Fragment (p/n 012-0105). Lane 4: Rat IgM Whole Molecule (p/n 012-0107). Lane 5: Rat Serum (p/n D310-05). All samples were reduced. Load: 50 ng per lane. Block: MB-070 for 30 min at RT. Primary Antibody: Anti-Rat IgG (H&L) (GOAT) Antibody (p/n 612-1102) 1:1,000 for 60 min at RT. Secondary Antibody: Anti-Goat IgG (DONKEY) Peroxidase Conjugated Antibody (p/n CUST10) 1:40,000 in MB-070 for 30 min at RT. Predicted/Observed Size: 25 and 55 kDa for Rat IgG and Serum, 25 kDa for F(c) and Fab, 78 and 25 kDa for IgM. Rat F(c) migrates slightly higher.