Anti-Beta Amyloid Antibody was produced by repeated immunizations with synthetic peptide corresponding to amino acid residues from the C-terminal region.
Anti-Beta Amyloid antibody is tested for use in ELISA, Western Blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 115 kDa in size corresponding to the APP protein in the appropriate cell lysat
Western Blot of Rabbit Anti-Beta Amyloid antibody. Lane 1: rat hippocampal lysate. Lane 2: none. Load: 10 µg per lane. Primary antibody: Beta Amyloid antibody at 1:400 for overnight at 4C. Secondary antibody: IRDye800(TM) rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 115 kDa, ~115kDa for APP protein. Other band(s): none.
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