CaM Kinase II phospho T286 Antibody, Rabbit, Polyclonal

Product Overview

• Article Name: CaM Kinase II phospho T286 Antibody, Rabbit, Polyclonal
• Biozol Catalog Number: ROC-612-401-C71
• Supplier Catalog Number: 612-401-C71
• Alternative Catalog Number: ROC-612-401-C71
• Manufacturer: Rockland Immunochemicals
• Host: Rabbit
• Category: Antikörper
• Application: WB
• Species Reactivity: Mouse, Rat
• Immunogen: Anti-CaM Kinase II pT286 Antibody was produced by repeated immunizations with synthetic phospho-peptide corresponding to amino acid residues surrounding Thr286.
• Conjugation: Unconjugated
• Alternative Names: Calcium/calmodulin-dependent protein kinase (CaM kinase) II alpha, Camk2a protein

Product Properties

• Clonality: Polyclonal
• Concentration: Titrated value sufficient to run approximately 10 mini blots.
• NCBI: 25400
• UniProt: P11275
• Buffer: 0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5
• Form: Liquid
• Target: Rat
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: ELISA: 1:10,000, WB: 1:1000
• Application Notes: Anti-CaM Kinase II pT286 antibody is tested for use in ELISA and Western Blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 50 kDa in size corresponding to alpha CaM Kinase II and 60 kDa corre

Images

Western Blot of Rabbit anti- CaM Kinase II Thr286 Antibody. Lane 1: rat brain lysate. Lane 2: rat brain lysate incubated in lambda-Ptase (1200 units for 30 min). Load: 10 µg per lane. Primary antibody: CaM Kinase II Thr286 antibody at 1:400 for overnight at 4C. Secondary antibody: IRDye800(TM) rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: ~50kDa alpha- and the ~60kDa for beta-CaM Kinase II phosphorylated at Thr286. Other band(s): none.