Anti-GABA(A) Receptor alpha 5 Antibody was produced by repeated immunizations with recombinant fusion proteins from the cytoplasmic loop of the alpha 5 subunit of rat GABAA.
Anti-GABA(A) Receptor alpha 5 (Rabbit) antibody is tested for use in Western Blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 55 kDa in size corresponding to the alpha 5 subunit of the GABA(
Western Blot of Rabbit anti-GABAA Receptor a 5 Antibody. Lane 1: rat hippocampus. Lane 2: none. Load: 7 µg per lane. Primary antibody: GABAA-R antibody at 1:400 for overnight at 4C. Secondary antibody: IRDye800(TM) rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: ~55kDa/~55kDa for alpha5-subunit of the GABAA-R. Other band(s): none.
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