Anti-GluR1 pS831 Antibody was produced by repeated immunizations with a synthetic phospho-peptide corresponding to amino acid residues surrounding Ser 831.
Anti-GluR1 pS831 (Rabbit) antibody is tested for use in Western Blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 100 kDa in size corresponding to the GluR1 protein phosphorylated at Ser 831
Western Blot of Rabbit anti-GluR1-Subunit Ser831 Antibody. Lane 1: rat hippocampal lysate. Lane 2: rat hippocampal lysate incubated in lambda-Ptase (1200 units for 30 min). Load: 10 µg per lane. Primary antibody: GluR1 pS831 antibody at 1:400 for overnight at 4C. Secondary antibody: IRDye800(TM) rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 100 kDa, 100 kDa for GluR1 pS831. Other band(s): none.
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