GluR1 phospho S845 Antibody, Rabbit, Polyclonal

Catalog Number: ROC-612-401-C83
Article Name: GluR1 phospho S845 Antibody, Rabbit, Polyclonal
Biozol Catalog Number: ROC-612-401-C83
Supplier Catalog Number: 612-401-C83
Alternative Catalog Number: ROC-612-401-C83
Manufacturer: Rockland Immunochemicals
Host: Rabbit
Category: Antikörper
Application: IHC, WB
Species Reactivity: Mouse, Rat
Immunogen: Anti-GluR1 pS845 Antibody was produced by repeated immunizations with synthetic phospho-peptide corresponding to amino acid residues surrounding Ser845.
Conjugation: Unconjugated
Alternative Names: Glutamate receptor 1, GluR1, AMPA-selective glutamate receptor 1, GluR-A, GluR-K1, Glutamate receptor ionotropic, AMPA 1
Clonality: Polyclonal
Concentration: Titrated value sufficient to run approximately 10 mini blots.
NCBI: 50592
UniProt: P19490
Buffer: 0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5
Form: Liquid
Target: Rat
Antibody Type: Primary Antibody
Application Dilute: ELISA: 1:1000 - 10,000, IHC: 1:1000, IF Microscopy: 1:200, WB: 1:1000
Application Notes: Anti-GluR1 pS845 antibody is tested for use in IHC and Western Blotting and suitable for IF and ELISA. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 100 kDa in size corresponding to GluR1 protein p
Western Blot of Rabbit GluR1-Subunit Ser845 Antibody. Lane 1: rat hippocampal lysate. Lane 2: rat hippocampal lysate incubated in lambda-Ptase (1200 units for 30 min). Load: 10 µg per lane. Primary antibody: GluR1 Ser845 antibody at 1:400 for overnight at 4C. Secondary antibody: IRDye800(TM) rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: ~100kDA/~100kDA for GluR1 protein phosphorylated at Ser845. Other band(s): none.