14-3-3 Antibody, Rabbit, Polyclonal

Product Overview

• Article Name: 14-3-3 Antibody, Rabbit, Polyclonal
• Biozol Catalog Number: ROC-612-401-D01
• Supplier Catalog Number: 612-401-D01
• Alternative Catalog Number: ROC-612-401-D01
• Manufacturer: Rockland Immunochemicals
• Host: Rabbit
• Category: Antikörper
• Application: WB
• Species Reactivity: Rat
• Immunogen: Anti-14-3-3 Antibody was produced by repeated immunizations with synthetic phospho-peptide corresponding to amino acid residues from the C-terminal region.
• Conjugation: Unconjugated
• Alternative Names: 14-3-3 protein beta/alpha, Prepronerve growth factor RNH-1, Protein kinase C inhibitor protein 1, KCIP-1, 14-3-3 protein beta/alpha N-terminally processed, Ywhab

Product Properties

• Clonality: Polyclonal
• NCBI: 56011
• UniProt: P35213
• Buffer: 0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5
• Form: Liquid
• Target: Rat
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: WB: 1:1000
• Application Notes: 14-3-3 antibody is tested for use in Western Blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 29 kDa in size corresponding to 14-3-3 protein in the appropriate cell lysate or extract.

Images

Western Blot of Rabbit Anti-14-3-3 Protein antibody. Lane 1: rat hippocampal lysate. Lane 2: none. Load: 10 µg per lane. Primary antibody: 14-3-3 antibody at 1:400 for overnight at 4C. Secondary antibody: IRDye800(TM) rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: ~29kDa, ~29kDa for 14-3-3 protein. Other band(s): none.