Anti-DARPP-32 pT75 Antibody was produced in rabbit by repeated immunizations with synthetic phospho-peptide corresponding to amino acid residues surrounding Thr75 conjugated to KLH.
Conjugation:
Unconjugated
Alternative Names:
Protein phosphatase 1 regulatory subunit 1B, Dopamine- and cAMP-regulated neuronal phosphoprotein
Anti-DARPP-32 pT75 Antibody is tested for use in ELISA and Western Blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 32 kDa in size corresponding to DARPP 32 protein phosphorylated at Thr75 i
Western Blot of Anti-DARPP-32 pT75 (Rabbit) Antibody. Lane 1: rat caudate lysate. Lane 2: rat caudate lysate incubated in lambda-Ptase (1200 units for 30 min). Load: 10 µg per lane. Primary antibody: DARPP-32 pT75 antibody at 1:400 for overnight at 4C. Secondary antibody: IRDye800(TM) rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: ~32kDa/~32kDa for DARPP-32 phosphorylated at Thr75. Other band(s): none.
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