MARCKS phospho S152/phospho S156 Antibody, Rabbit, Polyclonal

Product Overview

• Article Name: MARCKS phospho S152/phospho S156 Antibody, Rabbit, Polyclonal
• Biozol Catalog Number: ROC-612-401-D70
• Supplier Catalog Number: 612-401-D70
• Alternative Catalog Number: ROC-612-401-D70
• Manufacturer: Rockland Immunochemicals
• Host: Rabbit
• Category: Antikörper
• Application: WB
• Species Reactivity: Mouse, Rat
• Immunogen: Anti-MARCKS pS152/pS156 Antibody was produced in rabbit by repeated immunizations with synthetic phospho-peptide corresponding to amino acid residues surrounding Ser 152/156 conjugated to KLH.
• Conjugation: Unconjugated
• Alternative Names: Myristoylated alanine-rich C-kinase substrate, Protein kinase C substrate 80 kDa protein

Product Properties

• Clonality: Polyclonal
• NCBI: 3028
• UniProt: P30009
• Buffer: 0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5
• Form: Liquid
• Target: Rat
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: WB: 1:1000
• Application Notes: Anti-MARCKS pS152/pS156 (Rabbit) antibody is tested for use in Western Blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 87 kDa in size corresponding to MARCKS protein phosphorylated at Ser 1

Images

Western Blot of Rabbit anti-MARCKS pS152/pS156 antibody. Lane 1: rat brain lysate. Lane 2: rat brain lysate incubated in lambda-Ptase (1200 units for 30 min). Load: 10 µg per lane. Primary antibody: MARCKS pS152/pS156 antibody at 1:400 for overnight at 4C. Secondary antibody: IRDye800(TM) rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 87 kDa, 87 kDa for MARCKS pS152/pS156. Other band(s): none.