mGluR5/1a Antibody, Rabbit, Polyclonal

Product Overview

• Article Name: mGluR5/1a Antibody, Rabbit, Polyclonal
• Biozol Catalog Number: ROC-612-401-D77
• Supplier Catalog Number: 612-401-D77
• Alternative Catalog Number: ROC-612-401-D77
• Manufacturer: Rockland Immunochemicals
• Host: Rabbit
• Category: Antikörper
• Application: IHC, WB
• Species Reactivity: Mouse, Rat
• Immunogen: Anti-mGluR5/1a Antibody was produced in rabbit by repeated immunizations with synthetic peptide corresponding to amino acid residues from the C-terminal region conjugated to KLH.
• Conjugation: Unconjugated
• Alternative Names: mGluR5, Metabotropic Glutamate Receptor 5/1a

Product Properties

• Clonality: Polyclonal
• NCBI: 24418
• UniProt: P31424
• Buffer: 0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5
• Form: Liquid
• Target: Rat
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: IHC: 1:500, WB: 1:1000
• Application Notes: Anti-mGluR5/1a (Rabbit) antibody is tested for use in Western Blotting and IHC. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 125 kDa monomer and 250 kDa dimer in size corresponding to mGluR5 and m

Images

Western Blot of Rabbit Anti-metabotropic glutamate receptors (mGluR) 5/1a antibody. Lane 1: HEK 293 cells expressing mGluR5. Lane 2: HEK 293 cells expressing mGluR1a. Load: 10 µg per lane. Primary antibody: mGluR5/1a antibody at 1:400 for overnight at 4C. Secondary antibody: IRDye800(TM) rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: ~125kDa monomer and the ~250kDa dimers of mGluR1a and mGluR5. Other band(s): none.