Anti-Rab 3 Antibody was produced in rabbit by repeated immunizations with synthetic peptide corresponding to amino acid residues from the N-terminal region conjugated to KLH.
Anti-Rab 3 (Rabbit) antibody is tested for use in Western Blots. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 27 kDa in size corresponding to Rab3 protein in the appropriate cell lysate or extract
Western Blot of Rabbit anti-Rab 3 antibody. Lane 1: rat cortex lysate. Lane 2: none. Load: 10 µg per lane. Primary antibody: Rab 3 antibody at 1:1,000 for overnight at 4C. Secondary antibody: IRDye800(TM) rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 27 kDa for Rab 3. Other band(s): none.
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