Anti-TrkB Antibody was produced in rabbit by repeated immunizations with synthetic peptide corresponding to amino acid residues from the C-terminal region of TrkB.
Anti-Trk B antibody is tested for use in Western Blotting. Expect a band of approximately 93 kDa in size corresponding to TrkB protein in the appropriate cell lysate or extract. Researchers should determine optimal titers for applications that are not st
Western Blot of Rabbit anti-TrkB antibody. Lane 1: Rat Synaptic Membrane. Lane 2: None. Load: 10 µg per lane. Primary antibody: TrkB antibody at 1:1,000 for overnight at 4C. Secondary antibody: IRDye800(TM) rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 93 kDa for TrkB. Other band(s): TrkB splice variants and isoforms.
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