Anti-Tryptophan Hydroxylase 2 pS19 Antibody was produced in rabbit by repeated immunizations with synthetic phospho-peptide corresponding to amino acid residues surrounding Ser19 of Tryptophan Hydroxylase.
Anti-Trytophan Hydroxylase2 pS19 (Rabbit) antibody is tested for use in Western Blotting. Expect a band of approximately 55 kDa in size corresponding to tryptophan hydroxylase protein phosphorylated at Ser 19 in the appropriate cell lysate or extract. Re
Western Blot of Rabbit anti-Tryptophan Hydroxylase pS19 antibody. Lane 1: Recombinant tryptophan hydroxylase incubated in the absence of Ca2+/calmodulin dependent kinase II (Control). Lane 2: Recombinant tryptophan hydroxylase incubated in the presence of Ca2+/calmodulin dependent kinase II (CaMKII). Load: 10 µg per lane. Primary antibody: Tryptophan Hydroxylase pS19 antibody at 1:1,000 for overnight at 4C. Secondary antibody: IRDye800(TM) rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 55 kDa for Tryptophan Hydroxylase pS19. Other band(s): none.
* VAT and and shipping costs not included. Errors and price changes excepted