Rabbit IgG anti-Rat IgG (F(ab)2)-unconj., MinX none

Product Overview

• Article Name: Rabbit IgG anti-Rat IgG (F(ab)2)-unconj., MinX none
• Biozol Catalog Number: ROC-612-4104
• Supplier Catalog Number: 612-4104
• Alternative Catalog Number: ROC-612-4104
• Manufacturer: Rockland Immunochemicals
• Host: Rabbit
• Category: Antikörper
• Application: ELISA,IHC,WB
• Species Reactivity: Rat
• Immunogen: Rat IgG F(ab)2 fragment
• Conjugation: Unconjugated
• Alternative Names: Rabbit Anti Rat IgG F(ab)2 Antibody, Rabbit Anti-Rat IgG Fab2 Antibody, Rabbit Anti-Rat IgG Fab2 Fragment Antibody
• Format: IgG
• Target Specificity: IgG (F(ab')2)
• Cross-Adsorption (MinX): no cross-adsorbtion

Product Properties

• Clonality: Polyclonal
• Concentration: 7.3 mg/mL by UV absorbance at 280 nm
• Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Liquid (sterile filtered)
• Target: Rat
• Antibody Type: Secondary Antibody

Application Notes

• Application Dilute: ELISA: 1:20,000 - 1:100,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000
• Application Notes: Anti-Rat IgG F(ab)2 antibody has been tested by ELISA and western blot and is suitable for use in immunohistochemistry. Specific conditions for reactivity should be optimized by the end user.

Images

Western Blot of Anti-Rat IgG F(ab)2 (RABBIT) Antibody (p/n 612-4104). Lane M: 3 µl Molecular Ladder. Lane 1: Rat IgG whole molecule (p/n 012-0102). Lane 2: Rat IgG F(c) Fragment (p/n 012-0103). Lane 3: Rat IgG Fab Fragment (p/n 012-0105). Lane 4: Rat IgM Whole Molecule (p/n 012-0107). All samples were reduced. Load: 50 ng per lane. Block: MB-070 for 30 min at RT. Primary Antibody: Anti-Rat IgG F(ab)2 (RABBIT) Antibody (p/n 612-4104) 1:1,000 for 60 min at RT. Secondary Antibody: Anti-Rabbit IgG (GOAT) Peroxidase Conjugated Antibody (p/n 611-103-122) 1:40,000 in MB-070 for 30 min at RT. Predicted/Observed Size: 25 and 55 kDa for Rat IgG, 25 kDa for F(c) and Fab, 78 and 25 kDa for IgM. Rat F(c) migrates slightly higher.