Rabbit IgG anti-Rat IgM (µ)-FITC, MinX none

Product Overview

• Article Name: Rabbit IgG anti-Rat IgM (µ)-FITC, MinX none
• Biozol Catalog Number: ROC-612-4207
• Supplier Catalog Number: 612-4207
• Alternative Catalog Number: ROC-612-4207
• Manufacturer: Rockland Immunochemicals
• Host: Rabbit
• Category: Antikörper
• Application: FLISA,FACS,IF
• Species Reactivity: Rat
• Immunogen: Rat IgM whole molecule
• Conjugation: FITC
• Alternative Names: Rabbit Anti-Rat IgM (mu chain) Antibody fluorescein Conjugated, Rabbit Anti-Rat IgM mu Antibody FITC Conjugation
• Format: IgG
• Target Specificity: IgM (µ)
• Cross-Adsorption (MinX): no cross-adsorbtion

Product Properties

• Clonality: Polyclonal
• Concentration: 1.5 mg/mL by UV absorbance at 280 nm
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Lyophilized
• Target: Rat
• Antibody Type: Secondary Antibody

Application Notes

• Application Dilute: FLISA: 1:10,000 - 1:50,000, Flow Cytometry: 1:500 - 1:2,500, IF Microscopy: 1:1,000 - 1:5,000
• Application Notes: Anti-Rat IgM fluorescein conjugated antibody has been tested by western blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis

Images

Western Blot of Anti-Rat IgM (mu chain) (RABBIT) Antibody (p/n 612-4107). Lane M: 3 µl Molecular Ladder. Lane 1: Rat IgG whole molecule (p/n 012-0102). Lane 2: Rat IgG F(c) Fragment (p/n 012-0103). Lane 3: Rat IgG Fab Fragment (p/n 012-0105). Lane 4: Rat IgM Whole Molecule (p/n 012-0107). Lane 5: Rat Serum (p/n D310-05). All samples were reduced. Load: 50 ng per lane. Block: MB-070 for 30 min at RT. Primary Antibody: Anti-Rat IgM (mu chain) (RABBIT) Antibody (p/n 612-4107) 1:2,000 for 60 min at RT. Secondary Antibody: Anti-Rabbit IgG (GOAT) Peroxidase Conjugated Antibody (p/n 611-103-122) 1:40,000 in MB-070 for 30 min at RT. Predicted/Observed Size: 25 and 55 kDa for Rat IgG and Serum, 25 kDa for F(c) and Fab, 78 and 25 kDa for IgM. Rat F(c) migrates slightly higher.