Rabbit IgG anti-Rat IgM (µ)-HRPO, MinX none

Product Overview

• Article Name: Rabbit IgG anti-Rat IgM (µ)-HRPO, MinX none
• Biozol Catalog Number: ROC-612-4307
• Supplier Catalog Number: 612-4307
• Alternative Catalog Number: ROC-612-4307
• Manufacturer: Rockland Immunochemicals
• Host: Rabbit
• Category: Antikörper
• Application: ELISA,IHC,WB
• Species Reactivity: Rat
• Immunogen: Rat IgM whole molecule
• Conjugation: HRPO
• Alternative Names: Rabbit Anti-Rat IgM (mu chain) Antibody peroxidase Conjugated, Rabbit Anti-Rat IgM mu Antibody HRP Conjugation
• Format: IgG
• Target Specificity: IgM (µ)
• Cross-Adsorption (MinX): no cross-adsorbtion

Product Properties

• Clonality: Polyclonal
• Concentration: 1.5 mg/mL by UV absorbance at 280 nm
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Lyophilized
• Target: Rat
• Antibody Type: Secondary Antibody

Application Notes

• Application Dilute: ELISA: 1:30,000 - 1:70,000, IHC: 1:500 - 1:2,500, WB: 1:1,000 - 1:5,000
• Application Notes: Anti-Rat IgM (mu chain) Peroxidase Conjugated antibody has been tested by ELISA and is suitable for ELISA, Western Blot and Immunohistochemistry. Antibody should be optimized by the end user for specific reactive conditions.

Images

ELISA Results of Rabbit Anti-Rat IgM Antibody Peroxidase Conjugated tested against purified Rat IgM HRP. Each well was coated in duplicate with 1.0 µg of Rat IgM (p/n 012-0107). The working dilution is 1:62,800. The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using HRP Conjugate Stabilizer (p/n MB-076) and TMB substrate (p/n TMBE-1000).