Sheep IgG anti-Rat IgG (H+L)-FITC, MinX none

Product Overview

• Article Name: Sheep IgG anti-Rat IgG (H+L)-FITC, MinX none
• Biozol Catalog Number: ROC-612-6202
• Supplier Catalog Number: 612-6202
• Alternative Catalog Number: ROC-612-6202
• Manufacturer: Rockland Immunochemicals
• Host: Sheep
• Category: Antikörper
• Application: FLISA,FACS,IF
• Species Reactivity: Rat
• Immunogen: Rat IgG whole molecule
• Conjugation: FITC
• Alternative Names: Sheep Anti-Rat IgG fluorescein Conjugated Antibody, Sheep Anti-Rat IgG Antibody FITC Conjugation
• Format: IgG
• Target Specificity: IgG (H+L)
• Cross-Adsorption (MinX): no cross-adsorbtion

Product Properties

• Clonality: Polyclonal
• Concentration: 2.0 mg/mL by UV absorbance at 280 nm
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Lyophilized
• Target: Rat
• Antibody Type: Secondary Antibody

Application Notes

• Application Dilute: FLISA: 1:10,000 - 1:50,000, Flow Cytometry: 1:500 - 1:2,500, IF Microscopy: 1:1,000 - 1:5,000
• Application Notes: Anti-Rat IgG (H&L) fluorescein Antibody has been tested by ELISA and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including

Images

Western Blot of Anti-Rat IgG (H&L) (SHEEP) Antibody (p/n 612-6102). Lane M: 3 µl Molecular Ladder. Lane 1: Rat IgG whole molecule (p/n 012-0102). Lane 2: Rat IgG F(c) Fragment (p/n 012-0103). Lane 3: Rat IgG Fab Fragment (p/n 012-0105). Lane 4: Rat IgM Whole Molecule (p/n 012-0107). Lane 5: Rat Serum (p/n D310-05). All samples were reduced. Load: 50 ng per lane. Block: MB-070 for 30 min at RT. Primary Antibody: Anti-Rat IgG (H&L) (SHEEP) Antibody (p/n 612-6102) 1:1,000 for 60 min at RT. Secondary Antibody: Anti-Sheep IgG (DONKEY) Peroxidase Conjugated Antibody (p/n 613-703-168) 1:40,000 in MB-070 for 30 min at RT. Predicted/Observed Size: 25 and 55 kDa for Rat IgG and Serum, 25 kDa for F(c) and Fab, 78 and 25 kDa for IgM. Rat F(c) migrates slightly higher.