Sheep IgG anti-Rat IgG (H+L)-HRPO, MinX none

Product Overview

• Article Name: Sheep IgG anti-Rat IgG (H+L)-HRPO, MinX none
• Biozol Catalog Number: ROC-612-6302
• Supplier Catalog Number: 612-6302
• Alternative Catalog Number: ROC-612-6302
• Manufacturer: Rockland Immunochemicals
• Host: Sheep
• Category: Antikörper
• Application: ELISA,IHC,WB
• Species Reactivity: Rat
• Immunogen: Rat IgG whole molecule
• Conjugation: HRPO
• Alternative Names: Sheep Anti-Rat IgG peroxidase Conjugated Antibody, Sheep Anti-Rat IgG Antibody HRP Conjugation
• Format: IgG
• Target Specificity: IgG (H+L)
• Cross-Adsorption (MinX): no cross-adsorbtion

Product Properties

• Clonality: Polyclonal
• Concentration: 2.0 mg/mL by UV absorbance at 280 nm
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Lyophilized
• Target: Rat
• Antibody Type: Secondary Antibody

Application Notes

• Application Dilute: ELISA: 1:60,000 - 1:100,000, IHC: 1:500 - 1:2,500, WB: 1:1,000 - 1:10,000
• Application Notes: Anti-Rat IgG Peroxidase Conjugated Antibody has been tested by ELISA and is assayed against 1.0 µg of Rat IgG in a standard capture ELISA using ABTS (2,2-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code ABTS-100 as a substrate for 30 minutes at

Images

Western Blot of Anti-Rat IgG (H&L) (SHEEP) Antibody (p/n 612-6102). Lane M: 3 µl Molecular Ladder. Lane 1: Rat IgG whole molecule (p/n 012-0102). Lane 2: Rat IgG F(c) Fragment (p/n 012-0103). Lane 3: Rat IgG Fab Fragment (p/n 012-0105). Lane 4: Rat IgM Whole Molecule (p/n 012-0107). Lane 5: Rat Serum (p/n D310-05). All samples were reduced. Load: 50 ng per lane. Block: MB-070 for 30 min at RT. Primary Antibody: Anti-Rat IgG (H&L) (SHEEP) Antibody (p/n 612-6102) 1:1,000 for 60 min at RT. Secondary Antibody: Anti-Sheep IgG (DONKEY) Peroxidase Conjugated Antibody (p/n 613-703-168) 1:40,000 in MB-070 for 30 min at RT. Predicted/Observed Size: 25 and 55 kDa for Rat IgG and Serum, 25 kDa for F(c) and Fab, 78 and 25 kDa for IgM. Rat F(c) migrates slightly higher.