Rabbit IgG anti-Sheep IgG (H+L)-unconj., MinX none

Product Overview

• Article Name: Rabbit IgG anti-Sheep IgG (H+L)-unconj., MinX none
• Biozol Catalog Number: ROC-613-4102
• Supplier Catalog Number: 613-4102
• Alternative Catalog Number: ROC-613-4102
• Manufacturer: Rockland Immunochemicals
• Host: Rabbit
• Category: Antikörper
• Application: ELISA,IHC,WB
• Species Reactivity: Sheep
• Immunogen: Sheep IgG whole molecule
• Conjugation: Unconjugated
• Alternative Names: Rabbit Anti-Sheep IgG Antibody, Rabbit Anti Sheep IgG Antibody
• Format: IgG
• Target Specificity: IgG (H+L)
• Cross-Adsorption (MinX): no cross-adsorbtion

Product Properties

• Clonality: Polyclonal
• Concentration: 2.0 mg/mL by UV absorbance at 280 nm
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Liquid (sterile filtered)
• Target: Sheep
• Antibody Type: Secondary Antibody

Application Notes

• Application Dilute: ELISA: 1:50,000 - 1:200,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000
• Application Notes: Anti-Sheep IgG antibody has been tested by ELISA and is suitable for use in ELISA, lateral flow, immunohistochemistry, and western blot. Specific conditions for reactivity should be optimized by the end user.

Images

ELISA results of Rabbit Anti-Sheep IgG Antibody tested against purified Sheep IgG protein. Each well was coated in duplicate with 10 µg of Sheep IgG (p/n 013-0102). The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. The titer is 1:60,800. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 1% fish gelatin as blocking buffer, Goat Anti-Rabbit IgG Peroxidase (p/n 611-103-122), and TMB substrate p/n TMBE-1000.