Rabbit IgG anti-Sheep IgG (Fc)-unconj., MinX none

Product Overview

• Article Name: Rabbit IgG anti-Sheep IgG (Fc)-unconj., MinX none
• Biozol Catalog Number: ROC-613-4103
• Supplier Catalog Number: 613-4103
• Alternative Catalog Number: ROC-613-4103
• Manufacturer: Rockland Immunochemicals
• Host: Rabbit
• Category: Antikörper
• Application: ELISA,IHC,WB
• Species Reactivity: Sheep
• Immunogen: Anti-Sheep IgG was produced by repeated immunization with sheep IgG F(c) fragment in rabbit.
• Conjugation: Unconjugated
• Alternative Names: Rabbit Anti-Sheep IgG F(c) Antibody, Rabbit Anti-Sheep IgG Fc Antibody, Rabbit Anti-Sheep IgG Fc Fragment Antibody
• Format: IgG
• Target Specificity: IgG (Fc)
• Cross-Adsorption (MinX): no cross-adsorbtion

Product Properties

• Clonality: Polyclonal
• Concentration: 2.0 mg/mL by UV absorbance at 280 nm
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Liquid (sterile filtered)
• Target: Sheep
• Antibody Type: Secondary Antibody

Application Notes

• Application Dilute: ELISA: 1:10,000 - 1:200,000, IHC: 1:500 - 1:2,500, WB: 1:1,000 - 1:5,000
• Application Notes: Anti-Sheep IgG F(c) antibody has been tested by ELISA and dot blot and is assayed against 1.0 ug of Sheep IgG in a standard capture ELISA using ABTS (2,2-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code ABTS-100 as a substrate for 30 minutes at

Images

ELISA Results of Rabbit Anti-Sheep IgG F(c) Antibody tested against purified Sheep IgG F(c). Each well was coated in duplicate with 1.0 µg of Sheep IgG F(c) (p/n 013-0103) [Red Line] and Sheep IgG (p/n 013-01020 [Green Line]. The working dilution of Sheep IgG F(c) is 1:45,000. The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using HRP Conjugation Stabilizer (p/n MB-076), Goat Anti-Rabbit IgG HRP conjugated (p/n 611-103-122) and TMB substrate (p/n TMBE-1000).