Rabbit IgG anti-Sheep IgG (H+L)-unconj., MinX Hu, Polyclonal , Unconjugated

Product Overview

• Article Name: Rabbit IgG anti-Sheep IgG (H+L)-unconj., MinX Hu, Polyclonal , Unconjugated
• Biozol Catalog Number: ROC-613-4128
• Supplier Catalog Number: 613-4128
• Alternative Catalog Number: ROC-613-4128
• Manufacturer: Rockland Immunochemicals
• Host: Rabbit
• Category: Antikörper
• Application: ELISA,IHC,WB
• Species Reactivity: Sheep
• Immunogen: Sheep IgG whole molecule
• Conjugation: Unconjugated
• Alternative Names: rabbit anti-Sheep IgG pre-adsorbed antibody
• Format: IgG
• Target Specificity: IgG (H+L)
• Cross-Adsorption (MinX): Human

Product Properties

• Clonality: Polyclonal
• Concentration: 1.4 mg/mL by UV absorbance at 280 nm
• Buffer: 0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2
• Form: Liquid (sterile filtered)
• Target: Sheep
• Antibody Type: Secondary Antibody

Application Notes

• Application Dilute: ELISA: 1:20,000 - 1:200,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000
• Application Notes: Anti-Sheep IgG antibody has been tested by ELISA and is suitable for use in ELISA, immunohistochemistry, and western blot. Specific conditions for reactivity should be optimized by the end user.

Images

ELISA Results of Rabbit Anti-Sheep IgG Antibody (Min X Human Serum Proteins) tested against purified Sheep IgG MX Hu. Each well was coated in duplicate with 1.0 µg of Sheep IgG (p/n 013-0102) [Green Line] and Human IgG (p/n 009-0102) [Blue Line]. The working dilution of Sheep IgG MX Hu is 1:71,000. The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using HRP Conjugation Stabilizer (p/n MB-076), Goat Anti-Rabbit IgG HRP conjugated (p/n 611-103-122) and TMB substrate (p/n TMBE-1000).