Rabbit IgG anti-Sheep IgG (Fc)-unconj., MinX Hu, Polyclonal , Unconjugated

Product Overview

• Article Name: Rabbit IgG anti-Sheep IgG (Fc)-unconj., MinX Hu, Polyclonal , Unconjugated
• Biozol Catalog Number: ROC-613-4129
• Supplier Catalog Number: 613-4129
• Alternative Catalog Number: ROC-613-4129
• Manufacturer: Rockland Immunochemicals
• Host: Rabbit
• Category: Antikörper
• Application: ELISA,IHC,WB
• Species Reactivity: Sheep
• Immunogen: Sheep IgG F(c) fragment
• Conjugation: Unconjugated
• Alternative Names: Rabbit Anti-Sheep IgG F(c) Antibody, Rabbit Anti-Sheep IgG Fc Antibody, Rabbit Anti-Sheep IgG Fc Fragment Antibody
• Format: IgG
• Target Specificity: IgG (Fc)
• Cross-Adsorption (MinX): Human

Product Properties

• Clonality: Polyclonal
• Concentration: 1.0 mg/mL by UV absorbance at 280 nm
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Liquid (sterile filtered)
• Target: Sheep
• Antibody Type: Secondary Antibody

Application Notes

• Application Dilute: ELISA: 1:20,000 - 1:100,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000
• Application Notes: Anti-Sheep IgG F(c) antibody has been tested by ELISA and is suitable for ELISA, western blot, and immunohistochemistry, as well as other assays requiring lot-to-lot consistency.

Images

ELISA Results of Rabbit Anti-Sheep IgG F(c) Antibody (Min X Human Serum Proteins) tested against purified Sheep IgG F(c) Min Hu. Each well was coated in duplicate with 1.0 µg of Sheep IgG F(c) (p/n 013-0103) [Red Line] and Human IgG (p/n 009-0102) [Green Line]. The working dilution of Sheep IgG F(c) is 1:16,300. The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using HRP Conjugate Stabilizer (p/n MB-076), Goat Anti-Rabbit IgG HRP conjugated (p/n 611-103-122) and TMB substrate (p/n TMBE-1000).