Rabbit IgG anti-Sheep IgG (H+L)-HRPO, MinX Hu, Polyclonal

Product Overview

• Article Name: Rabbit IgG anti-Sheep IgG (H+L)-HRPO, MinX Hu, Polyclonal
• Biozol Catalog Number: ROC-613-4328-0500
• Supplier Catalog Number: 613-4328-0500
• Alternative Catalog Number: ROC-613-4328-0500
• Manufacturer: Rockland Immunochemicals
• Host: Rabbit
• Category: Antikörper
• Application: ELISA,IHC,WB
• Species Reactivity: Sheep
• Immunogen: Sheep IgG whole molecule
• Conjugation: HRPO
• Alternative Names: Rabbit anti-Sheep IgG Peroxidase Conjugated Secondary Antibody, Rabbit anti-Sheep IgG Antibody HRP Conjugation
• Format: IgG
• Target Specificity: IgG (H+L)
• Cross-Adsorption (MinX): Human

Product Properties

• Clonality: Polyclonal
• Concentration: 1.0 mg/mL by UV absorbance at 280 nm
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Lyophilized
• Target: Sheep
• Antibody Type: Secondary Antibody

Application Notes

• Application Dilute: ELISA: 1:30,000 - 1:70,000, IHC: 1:250 - 1:1,500, WB: 1:1,000 - 1:10,000
• Application Notes: Anti-Sheep IgG Peroxidase Conjugated Antibody has been tested by ELISA and is suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic ass

Images

ELISA Results of Rabbit Anti-Sheep IgG Antibody Peroxidase Conjugate (Min X Human Serum Proteins) tested against purified Sheep IgG Min X Hu HRP. Each well was coated in duplicate with 1.0 µg of Sheep IgG (p/n 013-0102). The working dilution is 1:57000. The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody.