Sheep IgG (H&L) Antibody DyLight(TM) 549 Conjugated, DL549, Rabbit, Polyclonal

Product Overview

• Article Name: Sheep IgG (H&L) Antibody DyLight(TM) 549 Conjugated, DL549, Rabbit, Polyclonal
• Biozol Catalog Number: ROC-613-442-002
• Supplier Catalog Number: 613-442-002
• Alternative Catalog Number: ROC-613-442-002
• Manufacturer: Rockland Immunochemicals
• Host: Rabbit
• Category: Antikörper
• Species Reactivity: Sheep
• Immunogen: Sheep IgG whole molecule
• Conjugation: DL549
• Alternative Names: Rabbit Anti-Sheep IgG DyLight(TM) 549 Conjugated Antibody, Rabbit Anti-Sheep IgG Antibody DyLight(TM)549 Conjugation

Product Properties

• Clonality: Polyclonal
• Concentration: 1.0 mg/mL by UV absorbance at 280 nm
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Lyophilized
• Target: Sheep
• Antibody Type: Secondary Antibody

Application Notes

• Application Dilute: FLISA: >1:20,000, IF Microscopy: >1:5,000, WB: >1:10,000
• Application Notes: This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platfor

Images

Analysis of coexpression of calretinin (CR), calbindin (CB), and NPY in GFP 1 cells in the cingulate cortex of FVBTg(GadGFP)45704Swn/J mice. A-E: Representative confocal images of cells in layers II-III of the cingulate cortex immunopositive for GFP (green), NPY (red), CR (white), and CB (blue). The solid yellow arrow indicates GFP1/CR1/CB1/NPY 1 cells, the open yellow arrow GFP1/CR2/CB2/NPY2 cells, and the open yellow arrowhead GFP1/CR1/CB1/NPY2 cells. F: Mean 6 standard deviation of relative numbers of GFP1/CR2/CB2/NPY2 cells (a), GFP1/CR1/CB2/NPY2 cells (b), GFP1/CR2/CB1/NPY2 cells (c), GFP1/CR1/ CB1/NPY2 cells (d), GFP1/CR1/CB2/NPY 1 cells (e), and GFP1/CR1/CB1/NPY 1 cells (f). Scale bar 5 20 lm in E (applies to A-E). Figure 11. PMID: 26669716.

Dylight(TM) dyes can be used for two-color Western Blot detection with low background and high signal. Anti-tubulin was detected using a DyLight(TM) 549 conjugate. Anti-TNF? was detected using a DyLight(TM) 649 conjugate. The image was captured using the Typhoon(TM) 9410 Imaging System.

Analysis of neuropeptide Y (NPY) expressing interneurons in the cingulate cortex of FVB-Tg(GadGFP)45704Swn/J mice. A-D: Representative confocal images of cells immunopositive for GFP (green), somatostatin (SOM, white), and NPY (red), and merged image of all channels. Solid yellow arrows indicate GFP1/SOM1/NPY 1 cells and open yellow arrows GFP1/ SOM1/NPY2 cells. E: Mean 6 standard deviation of relative numbers of GFP1/SOM2/NPY2 cells, GFP1/SOM1/NPY2 cells, and GFP1/SOM1/NPY 1 cells in the cingulate cortex. Scale bar 5 20 lm in D (applies to A-D). Figure 10. PMID: 26669716.

Morphological variety of group I GIN. A-E,left panelConfocal z-stack images as maximum intensity projections of representative group I GIN. Scalebars: 50 µm.Right panelImmunolabeling of biocytin-injected cells for GFP (green), CB (white or blue), CR (white or blue), SOM (white) or NPY (red). Fluorescence (white, GFP) and infrared (IR)-DIC (grey) images of recorded cells were acquired prior recording. Fig 8. PMID: 30001424.

Morphological varieties in group II GIN. Many group I GIN classified as Martinotti cells with massive axonal arborizations in layer 1 and in the home layer. All scalebars: 50 µm.A-F,left panelConfocal Z-stack images of biocytin-injected GIN as maximum intensity projections.Right panelCorresponding immunolabelings of the cells shown in theleft panel. Cells were labeled for GFP (green), CR (white or blue), CB (blue or white), SOM (white) or NPY (red). Fluorescence (GFP, white) and infrared-DIC (grey) images were acquired of cells in A-F prior recording. Fig 9. PMID: 30001424.