Donkey IgG anti-Sheep IgG (H+L)-unconj., MinX Ck,Gp,Hm,Ho,Hu,Ms,Rb,Rt, Polyclonal , Unconjugated

Product Overview

• Article Name: Donkey IgG anti-Sheep IgG (H+L)-unconj., MinX Ck,Gp,Hm,Ho,Hu,Ms,Rb,Rt, Polyclonal , Unconjugated
• Biozol Catalog Number: ROC-613-701-168
• Supplier Catalog Number: 613-701-168
• Alternative Catalog Number: ROC-613-701-168
• Manufacturer: Rockland Immunochemicals
• Host: Donkey
• Category: Antikörper
• Application: ELISA,IHC,WB
• Species Reactivity: Sheep
• Immunogen: Anti-Sheep IgG (H&L) was produced by repeated immunization with Sheep IgG whole molecule in donkey.
• Conjugation: Unconjugated
• Alternative Names: Donkey anti-Sheep IgG Antibody, Dk-a-Sheep IgG, Sheep Antibody in Donkey, Sheep Secondary Antibody
• Format: IgG
• Target Specificity: IgG (H+L)
• Cross-Adsorption (MinX): Gallus,Guinea pig,Hamster (all),Equine,Human,Mouse,Rabbit,Rat

Product Properties

• Clonality: Polyclonal
• Concentration: 1.0 mg/mL by UV absorbance at 280 nm
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Liquid (sterile filtered)
• Target: Sheep
• Antibody Type: Secondary Antibody

Application Notes

• Application Dilute: ELISA: 1:39,200 - 1:59,200, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000
• Application Notes: Anti-Sheep IgG (H&L) has been tested by ELISA and is suitable for use in immunoelectrophoresis, western-blot, competitive western-blot, ELISA and competitive ELISA assays. Specific conditions for reactivity and signal detection should be optimized by the

Images

ELISA Results of Donkey Anti-Sheep IgG Antibody (Min X Ch GP Ham Hs Hu Ms Rb Rt Serum Proteins) tested against purified Sheep IgG Mx8. Each well was coated in duplicate with 1.0 µg of Sheep IgG (p/n 013-0102) [Red Line], Chicken IgG (p/n 003-0102) [Green Line], Guinea Pig IgG (p/n 006-0102) [Blue Line], Hamster IgG (p/n 007-0102) [Purple Line], Horse IgG (p/n 008-0102), Human IgG (p/n 009-0102), Mouse IgG (p/n 010-0102), Rabbit IgG (p/n 011-0102), and Rat IgG (p/n 012-0102). The working dilution of Sheep IgG MX8 is 1:49,000. The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using HRP Conjugate Stabilizer (p/n MB-076), Goat Anti-Donkey IgG HRP conjugated (p/n 616-4302), and TMB substrate (p/n TMBE-1000).