Donkey IgG anti-Sheep IgG (H+L)-HRPO, MinX Ck,Gp,Hm,Ho,Hu,Ms,Rb,Rt, Polyclonal

Product Overview

• Article Name: Donkey IgG anti-Sheep IgG (H+L)-HRPO, MinX Ck,Gp,Hm,Ho,Hu,Ms,Rb,Rt, Polyclonal
• Biozol Catalog Number: ROC-613-703-168
• Supplier Catalog Number: 613-703-168
• Alternative Catalog Number: ROC-613-703-168
• Manufacturer: Rockland Immunochemicals
• Host: Donkey
• Category: Antikörper
• Application: ELISA,IHC,WB
• Species Reactivity: Sheep
• Immunogen: Anti-Sheep IgG (H&L) was produced by repeated immunization with Sheep IgG whole molecule in donkey.
• Conjugation: HRPO
• Alternative Names: Donkey Anti-Sheep IgG peroxidase Conjugated Antibody, Donkey Anti-Sheep IgG Antibody HRP Conjugation
• Format: IgG
• Target Specificity: IgG (H+L)
• Cross-Adsorption (MinX): Gallus,Guinea pig,Hamster (all),Equine,Human,Mouse,Rabbit,Rat

Product Properties

• Clonality: Polyclonal
• Concentration: 1.0 mg/mL by UV absorbance at 280 nm
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Lyophilized
• Target: Sheep
• Antibody Type: Secondary Antibody

Application Notes

• Application Dilute: ELISA: 1:65,000, IHC: 1:500 - 1:2,500, WB: 1:1,000 - 1:10,000
• Application Notes: Anti-Sheep IgG (H&L) peroxidase conjugated antibody has been tested by ELISA and is suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymat

Images

ELISA results of purified Donkey Anti-Sheep IgG peroxidase conjugated MX8 Antibody tested against purified Sheep IgG. Each well was coated in duplicate with 1.0 µg of Sheep IgG (green line). The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using TMB-1000 substrate.