Donkey IgG anti-Sheep IgG (H+L)-unconj., MinX none

Product Overview

• Article Name: Donkey IgG anti-Sheep IgG (H+L)-unconj., MinX none
• Biozol Catalog Number: ROC-613-7102
• Supplier Catalog Number: 613-7102
• Alternative Catalog Number: ROC-613-7102
• Manufacturer: Rockland Immunochemicals
• Host: Donkey
• Category: Antikörper
• Application: ELISA,IHC,WB
• Species Reactivity: Sheep
• Immunogen: Sheep IgG whole molecule
• Conjugation: Unconjugated
• Alternative Names: Donkey Anti-Sheep IgG Antibody
• Format: IgG
• Target Specificity: IgG (H+L)
• Cross-Adsorption (MinX): no cross-adsorbtion

Product Properties

• Clonality: Polyclonal
• Concentration: 2.0 mg/mL by UV absorbance at 280 nm
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Liquid (sterile filtered)
• Target: Sheep
• Antibody Type: Secondary Antibody

Application Notes

• Application Dilute: ELISA: 1:20,000 - 1:100,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000
• Application Notes: Anti-Sheep IgG secondary antibody has been tested by ELISA and is suitable for use in various immunoassays. Specific conditions for reactivity and signal detection should be optimized by the end user.

Images

ELISA results of purified ELISA of Donkey anti-Sheep IgG Antibody tested against purified Sheep IgG. Each well was coated in duplicate with 1.0 µg of Sheep IgG. The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using Blocking buffer MB-060-1000, Gt-a-Donkey IgG HRP conjugate 1:10,000, and TMB-8000 substrate.