Goat IgG anti-Swine IgG (H+L)-Biotin, MinX none

Product Overview

• Article Name: Goat IgG anti-Swine IgG (H+L)-Biotin, MinX none
• Biozol Catalog Number: ROC-614-1602
• Supplier Catalog Number: 614-1602
• Alternative Catalog Number: ROC-614-1602
• Manufacturer: Rockland Immunochemicals
• Host: Goat
• Category: Antikörper
• Application: ELISA,IHC,WB
• Species Reactivity: Porcine
• Immunogen: Swine IgG whole molecule
• Conjugation: Biotin
• Alternative Names: Goat Anti Swine IgG Biotin Conjugated Antibody, Goat Anti-Swine IgG Biotin Conjugated Antibody, Goat Anti-Swine IgG Antibody Biotin Conjugation
• Format: IgG
• Target Specificity: IgG (H+L)
• Cross-Adsorption (MinX): no cross-adsorbtion

Product Properties

• Clonality: Polyclonal
• Concentration: 2.0 mg/mL by UV absorbance at 280 nm
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Lyophilized
• Target: Swine
• Antibody Type: Secondary Antibody

Application Notes

• Application Dilute: ELISA: 1:20,000 - 1:100,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000
• Application Notes: Anti-Swine IgG (H&L) Biotin Conjugated Antibody has been tested by western blot and assayed against 1.0 ug of Swine IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin S000-03 and ABTS (2,2-azino-bis-[3-ethylbenthiazoline-6-sulfoni

Images

Western Blot of Biotin conjugated Goat anti-Swine antibody. Lane 1: Swine IgG. Lane 2: none. Load: 100 ng per lane. Primary antibody: Biotin conjugated swine antibody at 1:1,000 for overnight at 4C. Secondary antibody: HRP Streptavidin secondary antibody at 1:40,000 for 30 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 55 and 28 kDa for Swine IgG. Other band(s): none.