Rabbit IgG anti-Swine IgG (H+L)-HRPO, MinX none

Product Overview

• Article Name: Rabbit IgG anti-Swine IgG (H+L)-HRPO, MinX none
• Biozol Catalog Number: ROC-614-4302
• Supplier Catalog Number: 614-4302
• Alternative Catalog Number: ROC-614-4302
• Manufacturer: Rockland Immunochemicals
• Host: Rabbit
• Category: Antikörper
• Application: ELISA,IHC,WB
• Species Reactivity: Porcine
• Immunogen: Anti-Swine IgG whole molecule was produced by repeated immunization with Swine IgG whole molecule in rabbit.
• Conjugation: HRPO
• Alternative Names: Rabbit Anti-Swine IgG peroxidase Conjugated Antibody, Rabbit Anti-Swine IgG Antibody HRP Conjugation
• Format: IgG
• Target Specificity: IgG (H+L)
• Cross-Adsorption (MinX): no cross-adsorbtion

Product Properties

• Clonality: Polyclonal
• Concentration: 2.0 mg/mL by UV absorbance at 280 nm
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Lyophilized
• Target: Swine
• Antibody Type: Secondary Antibody

Application Notes

• Application Dilute: ELISA: 1:50,000 - 1:100,000, IHC: 1:500 - 1:2,500, WB: 1:1,000 - 1:5,000
• Application Notes: Anti-Swine IgG Peroxidase conjugated Antibody has been tested by ELISA and is suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic ass

Images

ELISA Results of Rabbit Anti-Swine IgG Antibody Peroxidase Conjugate tested against purified Swine IgG HRP. Each well was coated in duplicate with 1.0 µg of Swine IgG (p/n 014-0102). The working dilution is 1:112,000. The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using HRP Conjugate Stabilizer (p/n MB-076) and TMB substrate (p/n TMBE-1000).