Rabbit IgG anti-Swine IgG (H+L)-Alk. Phos., MinX none, ALP, Polyclonal , AP

Catalog Number: ROC-614-4502
Article Name: Rabbit IgG anti-Swine IgG (H+L)-Alk. Phos., MinX none, ALP, Polyclonal , AP
Biozol Catalog Number: ROC-614-4502
Supplier Catalog Number: 614-4502
Alternative Catalog Number: ROC-614-4502
Manufacturer: Rockland Immunochemicals
Host: Rabbit
Category: Antikörper
Application: ELISA,IHC,WB
Species Reactivity: Porcine
Immunogen: Swine IgG whole molecule
Conjugation: Alk. Phos.
Alternative Names: Rabbit Anti-Swine IgG alkaline phosphatase Conjugated Antibody, Rabbit Anti-Swine IgG Antibody alk phos Conjugation
Format: IgG
Target Specificity: IgG (H+L)
Cross-Adsorption (MinX): no cross-adsorbtion
Clonality: Polyclonal
Concentration: 1.0 mg/mL by UV absorbance at 280 nm
Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol, pH 8.0
Form: Liquid (sterile filtered)
Target: Swine
Antibody Type: Secondary Antibody
Application Dilute: ELISA: 1:14,000, IHC: 1:200 - 1:1,000, WB: 1:500 - 1:2,500
Application Notes: Anti-Swine IgG Alkaline Phosphatase Conjugated antibody has been tested by ELISA, dot blot, and western blot and is suitable for use in immunohistochemistry. Specific conditions for reactivity should be optimized by the end user.
Western Blot of Alkaline Phosphatase conjugated Rabbit anti-Swine IgG antibody. Lane 1: Swine IgG. Lane 2: none. Load: 100 ng per lane. Primary antibody: none. Secondary antibody: Alkaline Phosphatase swine secondary antibody at 1:1,000 for 60 min at RT. Block: MB-070 for 30 min RT. Predicted/Observed size: 55 kDa, 28 kDa for Swine IgG. Other band(s): none.