0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Form:
Liquid (sterile filtered)
Target:
Armenian Hamster
Antibody Type:
Secondary Antibody
Application Dilute:
ELISA: 1:20,000 - 1:40,000, ChIP: User Optimized, IHC: 1:1,000 - 1:5,000, IP: User Optimized, WB: 1:2,000 - 1:10,000
Application Notes:
Anti-Armenian Hamster IgG Antibody has been tested by ELISA and is suitable for immunoprecipitation, immunodiffusion, conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity.
ELISA Results of Goat Anti-Armenian Hamster IgG Antibody tested against purified Armenian Hamster IgG. Each well was coated in duplicate with 1.0 µg of Armenian Hamster IgG (p/n 019-001-002). The working dilution is 1:21,000. The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using HRP Conjugate Substrate (p/n MB-076), Donkey Anti-Goat IgG HRP conjugated (p/n 605-703-125), and TMB substrate (p/n TMBE-1000).
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