Anti-Hamster IgG HRP Secondary Antibody has been tested by ELISA and is designed for Western Blotting, ELISA and Immunohistochemistry. HRP conjugated secondary antibodies can also be used for a variety of other applications such as Assay Development.
ELISA results of purified Goat anti-Armenian Hamster IgG Antibody tested against purified Armenian Hamster IgG. Each well was coated in duplicate with 1.0 µg of Armenian Hamster IgG (p/n 019-001-002). The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gelatin as blocking buffer and TMB substrate p/n TMBE-1000.
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