Rabbit F(ab)2 anti-Dog IgG (H+L)-Biotin, MinX none

Product Overview

• Article Name: Rabbit F(ab)2 anti-Dog IgG (H+L)-Biotin, MinX none
• Biozol Catalog Number: ROC-704-406-002
• Supplier Catalog Number: 704-406-002
• Alternative Catalog Number: ROC-704-406-002
• Manufacturer: Rockland Immunochemicals
• Host: Rabbit
• Category: Antikörper
• Application: ELISA,IHC,WB
• Species Reactivity: Canine
• Immunogen: Dog IgG whole molecule
• Conjugation: Biotin
• Alternative Names: Rabbit F(ab)2 Anti-Dog Biotin Conjugated Antibody, Rabbit Fab2 Anti-Dog Antibody Biotin Conjugation, Rabbit Fab2 Anti-Dog Antibody Biotin Conjugation
• Format: F(ab')2
• Target Specificity: IgG (H+L)
• Cross-Adsorption (MinX): no cross-adsorbtion

Product Properties

• Clonality: Polyclonal
• Concentration: 1 mg/mL by UV absorbance at 280 nm
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Lyophilized
• Target: Dog
• Antibody Type: Secondary Antibody

Application Notes

• Application Dilute: ELISA: 1:20,000 - 1:100,000, IHC: 1:1,000-1:5,000, WB: 1:2,000-1:10,000
• Application Notes: F(ab)2 Anti-Dog IgG Biotin Antibody has been tested by ELISA and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including mult

Images

ELISA results of purified Rabbit F(ab)2 Anti-DOG IgG Antibody Biotin Conjugated tested against purified Dog IgG. Each well was coated in duplicate with 1.0 µg of Dog IgG (Blue Line). The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using Blocking buffer (p/n MB-060-1000), Streptavidin-HRP conjugated (p/n S000-03), and TMB substrate (p/n TMBE-1000).