Rabbit F(ab)2 anti-Hamster (all) IgG (H+L)-unconj., MinX none

Product Overview

• Article Name: Rabbit F(ab)2 anti-Hamster (all) IgG (H+L)-unconj., MinX none
• Biozol Catalog Number: ROC-707-401-002
• Supplier Catalog Number: 707-401-002
• Alternative Catalog Number: ROC-707-401-002
• Manufacturer: Rockland Immunochemicals
• Host: Rabbit
• Category: Antikörper
• Application: ELISA,IHC,WB
• Species Reactivity: Hamster (all)
• Immunogen: Golden Syrian Hamster IgG whole molecule
• Conjugation: Unconjugated
• Alternative Names: Rabbit F(ab)2 Anti-Golden Syrian & Armenian Hamster IgG Antibody, Rabbit F(ab)2 Anti-Hamster IgG Antibody, Rabbit Fab2 Anti-Hamster IgG Antibody
• Format: F(ab')2
• Target Specificity: IgG (H+L)
• Cross-Adsorption (MinX): no cross-adsorbtion

Product Properties

• Clonality: Polyclonal
• Concentration: 1.0 mg/mL by UV absorbance at 280 nm
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Liquid (sterile filtered)
• Target: Golden Syrian Hamster
• Antibody Type: Secondary Antibody

Application Notes

• Application Dilute: ELISA: 1:20,000 - 1:100,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000
• Application Notes: F(ab)2 Anti-Golden Syrian Hamster IgG Antibody has been tested by dot blot and is suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F

Images

ELISA results of purified Rabbit F(ab)2 Anti-Golden Syrian Hamster IgG Antibody tested against purified Hamster IgG (Green Line). Each well was coated in duplicate with 1.0 µg of Hamster IgG (p/n 007-0102). The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using Blocking buffer (p/n MB-060-1000), Goat Anti-Rabbit HRP conjugated (p/n 611-103-122), and TMB substrate (p/n TMBE-1000).