Goat F(ab)2 anti-Mouse IgG (H+L)-HRPO, MinX none

Catalog Number: ROC-710-1302
Article Name: Goat F(ab)2 anti-Mouse IgG (H+L)-HRPO, MinX none
Biozol Catalog Number: ROC-710-1302
Supplier Catalog Number: 710-1302
Alternative Catalog Number: ROC-710-1302
Manufacturer: Rockland Immunochemicals
Host: Goat
Category: Antikörper
Application: ELISA,IHC,WB
Species Reactivity: Mouse
Immunogen: Mouse IgG whole molecule
Conjugation: HRPO
Alternative Names: Goat F(ab)2 Anti-Mouse IgG Antibody Peroxidase Conjugation, Goat Fab2 Anti-Mouse IgG HRP conjugated Antibody
Format: F(ab')2
Target Specificity: IgG (H+L)
Cross-Adsorption (MinX): no cross-adsorbtion
Clonality: Polyclonal
Concentration: 1.0 mg/mL by UV absorbance at 280 nm
Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Form: Lyophilized
Target: Mouse
Antibody Type: Secondary Antibody
Application Dilute: ELISA: 1:10,000 - 1:50,000, IHC: 1:500 - 1:2,500, WB: 1:1,000 - 1:10,000
Application Notes: F(ab)2 Anti-Mouse IgG (H&L) Peroxidase Antibody has been tested by ELISA and western blot and is suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody
Western blot of Peroxidase conjugated Goat F(ab)2 Anti-Mouse IgG secondary antibody. Lane 1: Mouse IgG. Lane 2: None. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: Peroxidase goat secondary antibody at 1:1,000 for 60 min at RT. Blocking: MB-070 for 30 min at RT. Observed/Predicted size: 25 & 55 kDa, 25 & 55 kDa for Mouse IgG. Other band(s): None.