Goat F(ab)2 anti-Mouse IgM (µ)-HRPO, MinX none

Product Overview

• Article Name: Goat F(ab)2 anti-Mouse IgM (µ)-HRPO, MinX none
• Biozol Catalog Number: ROC-710-1307
• Supplier Catalog Number: 710-1307
• Alternative Catalog Number: ROC-710-1307
• Manufacturer: Rockland Immunochemicals
• Host: Goat
• Category: Antikörper
• Application: ELISA,IHC,WB
• Species Reactivity: Mouse
• Immunogen: Mouse IgM whole molecule
• Conjugation: HRPO
• Alternative Names: Goat F(ab)2 Anti-Mouse IgM (mu chain) Antibody Peroxidase Conjugation, Goat Fab2 Anti-Mouse IgM mu chain PE Conjugated Antibody
• Format: F(ab')2
• Target Specificity: IgM (µ)
• Cross-Adsorption (MinX): no cross-adsorbtion

Product Properties

• Clonality: Polyclonal
• Concentration: 1.0 mg/mL by UV absorbance at 280 nm
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Lyophilized
• Target: Mouse
• Antibody Type: Secondary Antibody

Application Notes

• Application Dilute: ELISA: 1:25,000 - 1:50,000, IHC: 1:500 - 1:2,500, WB: 1:2,000 - 1:10,000
• Application Notes: F(ab)2 Anti-Mouse IgM mu heavy chain peroxidase conjugated antibody has been tested by ELISA and is suitable for use in immunoelectrophoresis, western-blot, competitive western-blot, ELISA and competitive ELISA assays. Specific conditions for reactivity

Images

ELISA Results of F(ab)2 Goat Anti-Mouse IgM Antibody Peroxidase Conjugated. Each well was coated in duplicate with 1.0 µg of Mouse IgM (p/n 010-0107). The working dilution is 115,000. The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using TMB substrate p/n TMBE-1000.