Goat F(ab)2 anti-Mouse IgG (F(ab)2)-HRPO, MinX Bo,Ho,Hu,Rb,Rt,Sh, Polyclonal

Catalog Number: ROC-710-1331
Article Name: Goat F(ab)2 anti-Mouse IgG (F(ab)2)-HRPO, MinX Bo,Ho,Hu,Rb,Rt,Sh, Polyclonal
Biozol Catalog Number: ROC-710-1331
Supplier Catalog Number: 710-1331
Alternative Catalog Number: ROC-710-1331
Manufacturer: Rockland Immunochemicals
Host: Goat
Category: Antikörper
Application: ELISA,IHC,WB
Species Reactivity: Mouse
Immunogen: F(ab)2 Anti-Mouse IgG F(ab)2 fragment was produced by repeated immunization with Mouse IgG F(ab)2 fragment in goat.
Conjugation: HRPO
Alternative Names: Goat F(ab)2 Anti-Mouse IgG F(ab)2 Antibody peroxidase Conjugation, Goat Fab2 Anti-Mouse IgG Fab2 HRP Conjugated Antibody
Format: F(ab')2
Target Specificity: IgG (F(ab')2)
Cross-Adsorption (MinX): Bovine,Equine,Human,Rabbit,Rat,Sheep
Clonality: Polyclonal
Concentration: 1.0 mg/mL by UV absorbance at 280 nm
Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Form: Lyophilized
Target: Mouse
Antibody Type: Secondary Antibody
Application Dilute: ELISA: 1:130,000, IHC: 1:500 - 1:2,500, WB: 1:2,000 to 1:20,000
Application Notes: F(ab)2 Anti-Mouse IgG F(ab)2 fragment Peroxidase conjugated has been tested by ELISA and is suitable for use in immunoelectrophoresis, western-blot, competitive western-blot, ELISA and competitive ELISA assays. Specific conditions for reactivity and sign
ELISA Results of F(ab)2 Goat Anti-Mouse IgG F(ab)2 Antibody Peroxidase Conjugated Min X Bv, Hs, Hu, Rb, Rt, Sh Serum Proteins. Each well was coated in duplicate with 10 µg of Mouse IgG (p/n 010-0102). The working dilution is 193,000. The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% Fish Gel/PBS (p/n MB-066) and TMB substrate (p/n TMBE-1000).